Overall Reaction for the Pathway Glucose + 2 ADP + 2 Pi + 2 NAD+ ---> 2 Pyruvate + 2 ATP + 2 NADH + 2 H+ + 2 H2O a-D-Glucose a-D-Glucose-6-phosphate D-Fructose-6-phosphate D-Fructose-1,6-bisphosphate Glyceraldehyde-3-phosphate + Dihydroxyacetone phosphate Glyceraldehyde-3-phosphate 1,3-Bisphosphoglycerate 3-Phosphoglycerate 2-Phosphoglycerate Phosphoenolpyruvate Pyruvate Gibbs Free Energy Changes Rxn# Enzyme DG°'(kJ/mol) DG(kJ/mol) 1 Hexokinase -16.7 -33.5 2 Phosphoglucoisomerase +1.7 -2.5 3 Phosphofructokinase -14.2 -22.2 4 Aldolase +23.9 -1.3 5 Triose phosphate isomerase +7.6 +2.5 6 Glyceraldehyde-3-phosphatedehydrogenase +12.6 -3.4 7 Phosphoglycerate kinase -37.6 +2.6 8 Phosphoglycerate mutase +8.8 +1.6 9 Enolase +3.4 -6.6 10 Pyruvate kinase -62.8 -33.4 The DG values are estimated from intracellular concentrations of glycolytic intermediates in rabbit skeletalmuscle. N.B., the DG values after reaction number 5 are doubled since two G-3-P molecules exist after reaction number 5.
The DG values are estimated from intracellular concentrations of glycolytic intermediates in rabbit skeletalmuscle. N.B., the DG values after reaction number 5 are doubled since two G-3-P molecules exist after reaction number 5.
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