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The human genome project was a cinch compared to the complexity of understanding the extent
and type of gene expression in a given cell, the proteins produced, and how these proteins interact to create biological function.
The emerging field of proteomics, which will study these types of questions, will revolutionize our understanding of
normal and disease processes in cells. Richard Burgess, UW Madison, includes the following activities in proteomics:
(C&E New, July 31, 2000, pg 31)
- "Transcriptional profiling to determine which genes are transcribed into RNA in particular
cell type, developmental stage, or diseased state".
- High-throughput expression and purification of proteins
- Protein profiling, using 2D gel electrophoresis and mass spectrometry to study proteins
expressed in a cell
- Protein-protein interaction studies to see which proteins function together using the yeast
two hybrid method
- Pathway analysis to understand signal transduction and other complex cell processes
- Large scale protein folding and 3D structure studies
- Bioinformatics analysis of proteomic data
- More latter.
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